|
KMID : 0364820170530010029
|
|
Korean Journal of Microbiology
2017 Volume.53 No. 1 p.29 ~ p.38
|
|
|
Phylogenetic diversity of bacterial communities in a gray solar saltern and isolation of extremely halophilic bacteria using culturomics
|
|
Cho Geon-Yeong
Han Song-Ih
Whang Kyung-Sook
|
|
|
Abstract
|
|
|
|
In this study, we investigated the phylogenetic diversity of the bacterial community and isolation of extremely halophilic bacteria using culturomics in a gray solar saltern. The number of bacterial living cells, enumerated in a gray solar saltern by direct fluorescence microscopy was three to four orders of magnitude greater than those enumerated by plate counts, suggesting the distribution of ‘viable but non-culturable bacteria’. The biodiversity of bacterial communities in a gray solar saltern was investigated by pyrosequencing, 1,778 OTUs of bacteria were comprised of 18 phyla 46 classes 85 orders 140 families 243 genera with 6.16 diversity index. Archaea communities were composed of 3 phyla 6 classes 7 orders 7 families 38 genera with 4.95 diversity index from 643 OTUs. Totally 137 isolates were isolated by 59 different cultural methods based on culturomics considering culture media and conditions suitable for the growth of extremely halophilic bacteria. Phylogenetic analyses of extremely halophilic isolates based on 16S rRNA gene sequences, extremely halophilic isolates were composed of 4 phyla and 11 genera. Haloterrigena and Haloferax can be successfully isolated from culturomics. These culturomics were effective methods for collection of diversity of extremely halophilic bacteria.
|
|
|
KEYWORD
|
|
|
culturomics, extremely halophile, gray solar saltern, pyrosequencing
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|